Figure 3
Uptake of PLGA-coated Fe 3 O 4 microcapsules by MDA cells observed under fluorescence microscopy. (A) MDA cells after incubation with DiI-labeled PLGA-coated Fe3O4 microcapsules for 4 h followed by FITC staining for 45 min. The cytoplasm appears green. (B) MDA cells after staining with Hoechst for 30 min with the nucleus appearing blue. (C) DiI-labeled PLGA-coated Fe3O4 with a red appearance under fluorescence microscopy. (D) Merged images of (A), (B) and (C). (E) Appearance of microcapsules under light microscopy. (F) MDA cells after staining with FITC in the absence of PLGA particles. Scale bar: 10 μm.