Figure 2

Case14, lymph nodal involvement by MF. A: FNC smear of lymph nodal involvement by MF showing an atypical cell population of medium sized cells with dense coarse chromatin. In the background there are mature lymphocytes and numerous eosinophils (Diff Quik stain 270X). B: Atypical lymphoid cells with nuclear irregularities, with lymphocytes and eosinophils in the background. C: FC showing the complete absence of CD8 cells and loss of CD7 in the same CD3/CD2 positive cells. D: DHPLC Chromatogram of the TCRγ amplification product showing a single peak assessing the monoclonal status. Sanger electropherogram of the TCRγ amplification product from the same case showing one type of sequence. Blast analysis indicated the homology between this sequence and the germline Vg4-Jg1/2 rearrangement as in the IMGT® databases.