Figure 3

BORIS-specific shRNA knocks down BORIS and decreases MAGE-A1 gene expression. MDA-MB-468 (A, B, C) and MCF-7 (D, E) cells were transiently transfected with expression plasmid containing BORIS-specific shRNA and control plasmid encoding for a scramble shRNA. In contrast to MDA-MB-468 cells with their high levels of endogenous MAGE-A1 and BORIS mRNA levels, MCF-7 cells showing no expression of MAGE-A1 were additionally cotransfected with the expression plasmid containing the BORIS sequence. After a 48 hour transfection, mRNA levels were measured by PCR. Changes in mRNA expression levels of BORIS (A) and MAGE-A1 (B) by quantitative real-time PCR and MAGE-A1 by gel electrophoresis (C) in MDA-MB-468 cells. Real-time PCR derived changes in mRNA expression levels of BORIS (D) and MAGE-A1 (E) in MCF-7 cells. The significant p-values are shown.