STAT1C inhibits NF-κB signaling. Western blot results showed a detectable down-regulation of total p65 and phospho-p65 after STAT1C transfection in EC1 and EC109 cells (A). In the same experiment, nuclear/cytoplasmic fractionation studies showed that STAT1C induced a substantial decrease in nuclear p65 and phospho-p65 (B). (C) Using a NF-κB/luciferase reporter, we found that STAT1C gene transfection induce a significant down-regulation of the NF-κB transcription activity in ESCC cells transfected with STAT1C cells were harvested 48 hours after the gene transfection. (*p < 0.05) (E.V.: empty vector).