Figure 5
Effects of MDR-1 knockdown in ZR75-1 paclitaxel resistant cells A Western blot analysis of proteins extracted from the cell lines and probed with MDR1.Actin was used as a loading control. B. Western blot analysis of proteins extracted from ZR75-1 25PACR cells after transfections with MDR1 siRNA. Control cells were untreated, or with transfections reagents only or with siRNA targeting GAPDH; actin was used as a loading control. C. Graph shows the average IC50 of ZR75-1 25PACR cells after transfections with MDR1 siRNA. D. Graph shows the average IC50 of ZR75-1 50PACR cells after transfections with MDR1 siRNA. Asterisks indicate statistical difference * = p < 0.05, ** = p < 0.001.