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Table 1 Antibodies (AB), immunohistochemical techniques and resulting staining patterns

From: GLUT-1 expression is largely unrelated to both hypoxia and the Warburg phenotype in squamous cell carcinomas of the vulva

Antigen Epitope retrieval buffer Primary AB (Cat.-No., dilution) Primary AB supplier Detection system Staining pattern
GLUT-1 Citrate, pH 6.0 Cat.-No. GI817C01 (poly**), 1:400 DCS, Hamburg, Germany ImmPRESS Anti-Rabbit (Vector) Membranous
CA IX Citrate, pH 6.0 Cat.-No. 3829–1 (mono*), 1:400 Epitomics, Burlingame, CA ImmPRESS Anti-Rabbit (Vector) Membranous
CD34 Class II Tris/EDTA pH 9.0 Cat.-No. M7165 (mono*), 1:200 DAKO, Hamburg, Germany ImmPRESS Anti-Mouse (Vector) Membranous
Ki67 Citrate, pH 6.0 Cat.-No. ab16667 (mono*), 1:2000 Abcam, Cambridge, UK ImmPRESS Anti-Rabbit (Vector) Nuclear
aSMA (Klon IA4) Citrate, pH 6.0 Cat.-No. A1922C002 (mono*), 1:200 DCS, Hamburg, Germany ImmPRESS Anti-Mouse (Vector) Cytoplasmic
Hexokinase2 (C64G5) Citrate, pH 6.0 Cat.-No. 2867 (mono*), 1:200 Cell Signaling Technology, Danvers, MA ImmPRESS Anti-Rabbit (Vector) Cytoplasmic
PKM2 Citrate, pH 6.0 Cat.-No. 3198 (poly**), 1:100 Cell Signaling Technology, Danvers, MA ImmPRESS Anti-Rabbit (Vector) Cytoplasmic
  1. *monoclonal antibody, **polyclonal antibody.
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