Table 1 Antibodies (AB), immunohistochemical techniques and resulting staining patterns
Antigen | Epitope retrieval buffer | Primary AB (Cat.-No., dilution) | Primary AB supplier | Detection system | Staining pattern |
|---|---|---|---|---|---|
GLUT-1 | Citrate, pH 6.0 | Cat.-No. GI817C01 (poly**), 1:400 | DCS, Hamburg, Germany | ImmPRESS Anti-Rabbit (Vector) | Membranous |
CA IX | Citrate, pH 6.0 | Cat.-No. 3829–1 (mono*), 1:400 | Epitomics, Burlingame, CA | ImmPRESS Anti-Rabbit (Vector) | Membranous |
CD34 Class II | Tris/EDTA pH 9.0 | Cat.-No. M7165 (mono*), 1:200 | DAKO, Hamburg, Germany | ImmPRESS Anti-Mouse (Vector) | Membranous |
Ki67 | Citrate, pH 6.0 | Cat.-No. ab16667 (mono*), 1:2000 | Abcam, Cambridge, UK | ImmPRESS Anti-Rabbit (Vector) | Nuclear |
aSMA (Klon IA4) | Citrate, pH 6.0 | Cat.-No. A1922C002 (mono*), 1:200 | DCS, Hamburg, Germany | ImmPRESS Anti-Mouse (Vector) | Cytoplasmic |
Hexokinase2 (C64G5) | Citrate, pH 6.0 | Cat.-No. 2867 (mono*), 1:200 | Cell Signaling Technology, Danvers, MA | ImmPRESS Anti-Rabbit (Vector) | Cytoplasmic |
PKM2 | Citrate, pH 6.0 | Cat.-No. 3198 (poly**), 1:100 | Cell Signaling Technology, Danvers, MA | ImmPRESS Anti-Rabbit (Vector) | Cytoplasmic |