Figure 5

Glucose consumption and lactate production in NB4 and THP-1 under normoxia versus hypoxia. NB4 and THP-1 were cultured in the same condition as cell proliferation assay for 48 hours in triplicate. Then, concentration of glucose and lactic acid in the culture supernatant was measured by using D-Glucose kit or L-Lactic acid kit. Both cell lines showed increased glucose consumption under hypoxia compared with normoxia with statistical significance (p = 0.0013 in NB4, p = 0.0054 in THP-1) (A). THP-1 demonstrated increased lactate production under hypoxia (p < 0.0001), but NB4 did not (p = 0.0663) (B). H: under hypoxia, N: under normoxia. *** p < 0.001, ** p < 0.01, ns p > 0.05 (unpaired, two-tailed t-test).