Figure 4

HCI2509 induces apoptotic cell death. (A, B) Cell viability and caspase activation at 0, 24, 48, and 72 hours in (A) AN3CA and (B) KLE cells treated with 3X EC50 HCI2509. Measurements were normalized to their respective vehicle (0.3% DMSO) sample at the appropriate time point. (C, D) Fluorescence microscopy images of (C) AN3CA and (D) KLE cell lines after exposure to either vehicle or 3X EC50 HCI2509 and then stained with TUNEL for apoptotic nuclei (green), DAPI for nuclei (blue), and phalloidin for actin (red). HCI2509 treatment induced apoptosis with apoptotic cells marked with (*).