Table 1 Detailed aspects for each antibody used in immunohistochemistry

MCT1

Citrate buffer (0.01 M, pH = 6), 98°C, 20’

AB3538P

1:200, overnight

Chemicon International

MCT2

Citrate buffer (0.01 M, pH = 6), 98°C, 20’

sc-50322

1:200, 2 hours

Santa Cruz Biotechnology

MCT4

Citrate buffer (0.01 M, pH = 6), 98°C, 20’

sc-50329

1:500, 2 hours

Santa Cruz Biotechnology

CD147

EDTA (1 mM, pH = 8), 98°C, 20’

sc-71038

1:400, overnight

Santa Cruz Biotechnology

GLUT1

Citrate buffer (0.01 M, pH = 6), 98°C, 20’

ab15309-500

1:500, 2 hours

AbCam

CAIX

Citrate buffer (0.01 M, pH = 6), 98°C, 20’

ab15086

1:2000, 2 hours

AbCam

VEGF-A

CC1 (pH = 8,2) Ventana

VG-1

1:200, 60 minutes

AbCam

VEGF-C

ptLink (pH = 9) Dako

18-2255

1:200, 60 minutes

Invitrogen

VEGF-D

ptLink (pH = 9) Dako

ab63068

1:50, 60 minutes

AbCam

VEGFR-1

ptLink (pH = 9) Dako

ab9540

1:300, 60 minutes

AbCam

VEGFR-2

ptLink (pH = 9) Dako

ab2349

1:100, 60 minutes

AbCam

VEGFR-3

ptLink (pH = 9) Dako

ab72240

1:50, 60 minutes

AbCam