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Table 1 Detailed aspects for each antibody used in immunohistochemistry

From: Lactate transporters and vascular factors in HPV-induced squamous cell carcinoma of the uterine cervix

Protein Antigen retrieval Antibody Antibody dilution and incubation time
MCT1 Citrate buffer (0.01 M, pH = 6), 98°C, 20’ AB3538P 1:200, overnight
Chemicon International
MCT2 Citrate buffer (0.01 M, pH = 6), 98°C, 20’ sc-50322 1:200, 2 hours
Santa Cruz Biotechnology
MCT4 Citrate buffer (0.01 M, pH = 6), 98°C, 20’ sc-50329 1:500, 2 hours
Santa Cruz Biotechnology
CD147 EDTA (1 mM, pH = 8), 98°C, 20’ sc-71038 1:400, overnight
Santa Cruz Biotechnology
GLUT1 Citrate buffer (0.01 M, pH = 6), 98°C, 20’ ab15309-500 1:500, 2 hours
AbCam
CAIX Citrate buffer (0.01 M, pH = 6), 98°C, 20’ ab15086 1:2000, 2 hours
AbCam
VEGF-A CC1 (pH = 8,2) Ventana VG-1 1:200, 60 minutes
AbCam
VEGF-C ptLink (pH = 9) Dako 18-2255 1:200, 60 minutes
Invitrogen
VEGF-D ptLink (pH = 9) Dako ab63068 1:50, 60 minutes
AbCam
VEGFR-1 ptLink (pH = 9) Dako ab9540 1:300, 60 minutes
AbCam
VEGFR-2 ptLink (pH = 9) Dako ab2349 1:100, 60 minutes
AbCam
VEGFR-3 ptLink (pH = 9) Dako ab72240 1:50, 60 minutes
AbCam