Figure 2

Characterization of intra-tibial bone metastasis model of VCaP-Luc. (A) Measurement of intra-tibial tumor growth in intact (sham surgery) and castrated (castrated before intra-tibial injection of tumor cells) NSG mice by bioluminescence imaging once a week. Error bars represent SEM of n = 10 mice/group. (B) Faxitron and μCT imaging of intra-tibial PC model in intact mice. Faxitron imaging (in vivo) was performed at indicated time intervals during the study. The inset of Faxitron imaging shows control leg (no intra-tibial tumors) and tumor leg (received VCaP cell intra-tibially) at week 9. Ex vivo μCT imaging was performed and reconstructed at the termination of the study (week 9). Control leg and tumor legs after 3-D reconstruction is shown. (C) Faxitron and μCT imaging of intra-tibial PC model in Castrated mice. Faxitron imaging (in vivo) was performed at indicated time intervals during the study. The inset of Faxitron imaging shows control leg (no intra-tibial tumors) and tumor leg (received VCaP-Luc cells intra-tibially) at week 9. Ex vivo μCT imaging was performed and reconstructed at the termination of the study (week 9). Control leg and tumor legs after 3-D reconstruction is shown.