IL-6 and IL-8 expression in CD49f/CD24 sorted cell populations. A
E) dCt values obtained by subtracting 18S rRNA from gene of interest. dCt values are inversely proportional to expression. Black symbols: benign tissue samples (B), red symbols: IHC designated ER positive IDC tumor samples (+), blue symbols: IHC designated ER negative IDC tumor samples (−). Black bars indicate median values. B (IL-6) and F (IL-8) Fold change when sorted cell values were compared to tumor of origin. C (IL-6) and G (IL-8) Fold change when tumor sorted cell values were compared to averaged benign tissue values. D (IL-6) and H (IL-8) Fold change when sorted cell values from tumor samples were compared to averaged sorted cell values from benign tissue. (*, p-value <0.05).