Figure 3
ERA and ERB detection in CD49f/CD24 sorted cell populations. A-D) ERA expression, E-H) ERB expression. A, B) dCt values were obtained by subtracting 18S rRNA from gene of interest. dCt values are inversely proportional to expression. Black symbols: benign tissue samples (B), red symbols: IHC designated ER positive IDC tumor samples (+), blue symbols: IHC designated ER negative IDC tumor samples (−). In B-D and F-H Bars indicate values obtained when Fold change (RQ) values were calculated from averaged samples. Symbols indicate fold changes for individual data points. Black lines indicate median values. B, F) Fold change (RQ) when sorted cell values were compared to tumor of origin. C-G) Fold change (RQ) when sorted cell values from tumors were compared to averaged benign tissue values. D-H) Fold change (RQ) when sorted cell values from tumors were compared to averaged sorted cell values from benign tissue. (*, p-value <0.05 for each bracket).