Effect of NC on the expression of Bcl-2 and Bax in hepatic cancer xenograft mice and HepG2 cells. The mRNA levels of Bcl-2 and Bax in tumor tissues (A) or HepG2 cells (B) were determined by RT-PCR. The protein expression levels of Bcl-2 and Bax in tumor tissues (C) or HepG2 cells (D) were determined by Western blotting. GAPDH and β-actin were used as the internal controls for the RT-PCR or Western blotting, respectively. Images are representatives of 3 individual mouse in each group or of three independent cell-based experiments.