Effect of NC on cell proliferation in hepatic cancer xenograft mice and HepG2 cells. (A) Ki-67 assay in tumor tissues (400 ×). Data shown are averages with S.D. (error bars) from 6 individual mouse in each group. *P < 0.01, versus controls. (B) HepG2 cell colony formation assay. The data were normalized to the viability or survival of control cells (100%, treated with 0.1% DMSO vehicle). (C) HepG2 cell cycle was analyzed by FACS and the proportion of cells in S-phase was calculated. Data from panels B-D are averages with S.D. (error bars) from at least three independent experiments. *P < 0.01, versus control cells.