Figure 3

Sequence analysis of BRAF exon 15. Electropherograms from direct sequencing of the tumor’s genomic DNA (A), displaying an overlapping pattern starting from nucleotide 1799 (indicated by an arrow) and cloned PCR products (B) showing the wild type allele (left) and the c.1799_1803delinsAT mutation (right) that leads to amino acid substitution and deletion (p.V600_K601delinsD).