Expression of Kiss-1 and Kiss-1R in colorectal cancer. A. Immunohistochemical staining of Kiss-1 and Kiss-1R in normal colorectal tissue and tumour tissue; Arrows point to the staining of Kiss-1 and Kiss-1R in the cytoplasm of normal cells. Placenta was used as the positive control for both Kiss-1 and Kiss-1R. B. Semi quantitative analysis used to verify the protein expression level of Kiss-1 and Kiss-1R protein in normal background and tumour tissue. C. Kaplan- Meier survival analysis displaying relationship between the transcript levels of Kiss-1R and overall survival. D. Kaplan- Meier survival analysis displaying the relationship between the transcript levels of Kiss-1R and disease free survival. Patients with high levels of Kiss-1R had a significantly shorter overall survival being 85.8 months (95% C.I. 49.9- 121.6 months), p = 0.011 in comparison to those with low levels (143.0 months, 95% C.I. 124.261- 161.712). The patients with lower expression of Kiss-1R also had longer disease free survival (median = 133.2 months, 95% C.I. 111.7- 154.7 months), p = 0.033 compared with those with higher expression levels (median = 88.3 months, 95% C.I. 50.1- 126.6).