Figure 9
Cell cycle analysis. Cell cycle distribution of SK-HEP-1 ( A ), SK-sphere ( B ), HLE ( C ), and HLE-sphere ( D ) cells, respectively. DNA content of the cells was analyzed as described in Methods. Each panel ( A to D ) shows a representative histogram (upper) and a summary table (lower). E, The mRNA levels of P21 were measured with semi-quantitative RT-PCR. **P < 0.05 with the Mann-Whitney U-test.