Figure 2
Factors for sphere induction. Sphere induction was performed with a cell density of 1 × 105 SK-HEP-1 cells/mL for 7 days. A, complete sphere induction medium was used. B, C, D, and E, complete induction medium lacking bFGF, EGF, LIF, or NSF-1, respectively, was used. F, the basal medium was supplemented with only NSF-1.