Figure 6

The molecular changes on ADI treatment enhanced the sensitivity of PANC-1 cells to GEM. A, The mRNA levels of Bax, Bcl-2, caspase-3, and -9, and survivin were examined in four experimental groups of PANC-1 cells by qRT-PCR analyses. *, P < 0.05, as compared with the control group or indicated groups; NS, not significant. B, The ratio of nuclear p65 to cytoplasmic p65 protein expression, and the relative expression of p65 protein in nuclear extracts were tested in control, ADI, GEM, or ADI + GEM-treated PANC-1 cells. *, P < 0.05, as compared with the control group; ^#, P < 0.05, as compared with the indicated groups; NS, not significant. C, Inhibition of GEM-induced p65 nuclear translocation by ADI in PANC-1 cells was visualized by in situ immunofluorescence microscopy. D, The ratio of p-p65 (Ser536) to total (t)-p65 protein expression in nuclear and cytoplasmic extracts was tested in PANC-1 cells treated with experimental treatments. *, P < 0.05, as compared with the control group; ^#, P < 0.05, as compared with the indicated groups; NS, not significant. E, The phosphorylation levels of c-Jun protein (Ser73) in nuclear extracts of PANC-1 cells treated with various agents were detected by western blotting. *, P < 0.05, as compared with the control group; ^#, P < 0.05, as compared with the indicated groups; NS, not significant. F, The levels of IAP survivin protein in nuclear extracts from treated-PANC-1 cells were detected by western blotting. *, P < 0.05, as compared with the control group; ^#, P < 0.05, as compared with the indicated groups.