Figure 4

Correlation between miR-27a and PLK2 expression in LSCC. (A) Relative expression of PLK2 mRNA levels in the cell lines by qRT-PCR. The relative expression was calculated as the ratio of PLK2 mRNA level to the internal control using the equation RQ = 2^–ΔΔCT. (B) Relative expression of PLK2 protein levels in the cell lines by Western blot. The relative expression level in each group is indicated as the ratio of PLK2 to β-actin protein levels. (C) PLK2 protein levels in LSCC tissues by Western blot. Up: Representative images of Western blot result. T and R indicate cancer tissue and the paired adjacent tissue, respectively. Down: Statistical analysis of relative expression of PLK2 protein in each sample. The relative expression was calculated as the ratio of PLK2 protein level in cancer tissue to that in the paired adjacent tissue in each case. The digit on X-axis show the number of the paired samples. (D) Statistical analysis of the PLK2 protein expression in LSCC tissues. T and R indicate cancer tissue and the paired adjacent tissue, respectively. (E) Correlation between miR-27a and PLK2 expression in LSCC by Pearson’s product–moment correlation coefficient. Data were expressed as the mean ± SD from three independent experiments. P < 0.05 is indicated as symbol*.