Figure 1

miR-27a expression in LSCC tissues and Hep2 cells by qRT-PCR. (A) Relative expression of miR-27a in LSCC tissues. Y-axis indicates the ratio of relative miR-27a expression in cancer tissue to that in the paired adjacent tissue. The relative expression was calculated as the ratio of miR-27a to the internal control using the equation RQ = 2^–ΔΔCT in each sample. The digit on X-axis show the number of the paired samples used in the study. (B) Statistical analysis of miR-27a expression in LSCC. T and R indicate cancer tissue and the paired adjacent tissue, respectively. (C) Normalized box plot for miR-27a expression in LSCC. T and R indicate cancer tissue and the paired adjacent tissue, respectively. (D) miR-27a expression in the cell lines. Y-axis indicates relative miR-27a expression which was calculated as the ratio of miR-27a to the internal control using the equation RQ = 2^–ΔΔCT. Data were expressed as the mean ± SD from three independent experiments. P < 0.05 is indicated as symbol*.