Fibulin-1 suppressed bladder cancer
in vivo. Five mice were used per group conducted by 5637 cells transduced to express blank vehicle (Lenti-NC) or fibulin-1 (Lenti-FBLN1). A) Tumor growth curves of xenograft inoculated in nude mice. Curves represent average tumor size of 5 mice per time point. B) Representative images showed the xenograft tumors. C) Representative tumor sections of xenograft tumors derived from 5637 cells transduced to express Lenti-NC (a–c) and Lenti-FBLN1 (d–f). (a) and (d) show H&E stained images; (b) and (e), TUNEL of the xenograft tumors, white arrows showed the positive plots; (c) and (f), immunohistochemistry for CD31 (in dark brown color). D) Quantitative analyses of TUNEL are shown as mean ± SD of five randomly selected microscope fields per slice of mice in the two groups. E) The mean ± SD of blood vessel number of five randomly selected microscope fields per slice of mice in the two groups. Asterisk indicates p < 0.05.