Fibulin-1 functioned as a tumor suppressor in bladder cancer cells. The effect of ectopic FBLN1 expression on tumor cell proliferation was investigated by A) CCK-8 and B) EdU assay in 5637 and HT1376 cells. Data are plotted as the mean ± SD of 3 independent experiments relative to mock treatments. C) The effect of ectopic FBLN1 expression on tumor cell tumorgenesis was investigated by the monolayer colony-formation assay. Quantitative analyses of colony numbers are shown as mean ± SD. D) Fibulin-1 induced apoptosis of bladder cancer cells. The early stage apoptosis cells were detected for Annexin V-PE+/7-AAD-. Quantitative analyses of apoptotic cell numbers are shown in the right panel as values of mean ± SD. Asterisk indicates p < 0.05.