ZD55-AChE inhibited the growth of gastric cancer stem cells. (A) CD44+ AGS cells resisted to chemo-therapeutic drugs. Cells were treated with etoposide (5 μg/mL) or 5-FU (1 μg/mL) for 48 hours, and the cell viability were measured by MTT assay. (B) Ad.AChE inhibited CD44+ AGS cell growth as measured by MTT assay. (C) ZD55-AChE potently reduced the cell viability of CD44+ AGS cells as measured by MTT assay. CD44+ AGS cells were infected with the indicated MOI of adenovirus for 96 hours. MTT experiments were repeated 3 times. Data are shown as fold change relative to that of mock-treated cells. All data shown represent mean ± SD (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001.