Figure 7
Knockdown of HSP70/MRJ alters the expression of invasion related genes mmp2 and mmp9 using quantitative RT-PCR analysis. (A) Knockdown of uPAR decreased the mRNA levels of mmp2 and mmp9. Total RNAs were isolated from a pair of stably transfected cell lines (HEK 293-uPAR vs HEK293T cells) and from HEK 293-uPAR cells transiently transfected with psiuPAR or psiSC for 48 hours. The relative levels of the candidate genes (upar, upa, mmp2 and mmp9) were analyzed by quantitative RT-PCR (Q-PCR) analysis. (B) Knockdown of MRJ/HSP70 decreased mRNA levels of mmp2 and mmp9. Total RNAs were isolated from HEK 293-uPAR or HEK293T cells after transfected with psiSC, or psiMRJ, or psiHSP70, or psiMRJ plus psiHSP70 for 48 hours. The relative levels of the candidate genes (mrj, hsp70, upar, upa, mmp2 and mmp9) were analyzed by quantitative-PCR. The comprehensive inhibition ratio was calculated from the results of the mRNA level in HEK 293-uPAR cells divided by the mRNA level in HEK293T cells. The results represent three independent experiments with triplicate samples for each experiment *P < 0.05: significant different from the control.