Figure 4

2-24a/Cu decreases HSP90AA1 protein in U2os cells and HeLa cells. (A, B) Western blot analysis of HSP90AA1, its client proteins (PIM1, AKT1), and HSPA1A in U2os (A) and HeLa (B) cells, respectivity. U2os or HeLa were cultured with 0.1% DMSO, 10 μM 2-24a, 2 μM 2-24a/Cu or 5 μM 2-24a/Cu for 8 h. Cells were harvested and lysed for western blotting. (C, D) Western blot analysis of HSP90AA1 in U2os (C) and HeLa (D) cells treated with 5 μM 2-24a/Cu for different time. U2os and HeLa cells were cultured with 5 μM 2-24a/Cu for 0 h, 6 h, 12 h, and 18 h and then harvested and lysed for western blotting.