Correlation between ERK1 and STAT3 expression and chemoresistance in patients with conventional osteosarcoma

BMC Cancer

Table 2 Description of oligonucleotides and sequence parameters for QRT-PCR

Name gene	Oligo direct	Oligo reverse	PCR conditions	Cycle number	GeneInfo identifier
18S	CTACCACATCCAAGGAAGGCA	TTTTTCGTCACTACCTCCCCG	95°C 15 sec	35	124517659
18S	CTACCACATCCAAGGAAGGCA	TTTTTCGTCACTACCTCCCCG	67°C 30 sec	35	124517659
ERK1	CTAAAGCCCTCCAACCTGCT	CAGCCCACAGACCAGATGT	95°C 15 sec	45	158138506
ERK1	CTAAAGCCCTCCAACCTGCT	CAGCCCACAGACCAGATGT	60°C 30 sec	45	158138506
STAT3	AAAGTCAGGTTGCTGGTCAAA	TGCCGTTGTTGGATTCTTC	95°C 15 sec	45	76253927
STAT3	AAAGTCAGGTTGCTGGTCAAA	TGCCGTTGTTGGATTCTTC	60°C 30 sec	45	76253927

ISSN: 1471-2407