Figure 1
From: The impact of S6K1 kinase on neuroblastoma cell proliferation is independent of GLI1 signaling
S6K1 and GLI1 knockdown reduces SK-N-AS cellular proliferation. SK-N-AS cells, cultured for 48 hours following transfection with control (siCN), GLI1 (siGLI1) or S6K1 (siS6K1) siRNAs, were subjected to the EdU incorporation assay for 4 hours. The percentage of cells labeled with Alexa Fluor 488 azide was detected by flow cytometry. The data were analyzed with the one-way ANOVA test followed by Tukey’s multiple comparison using the GraphPad Prism software. Each bar represents the mean ± SEM of three independent experiments. *, Statistical significant, P < 0.05 compared to control. One representative experiment is shown in the histograph. Note that treatment with the S6K1 siRNAs is more effective than the GLI1 siRNAs in reducing cellular proliferation.