Figure 3

Clonogenic survival after treatment with gemcitabine or dFdU plus radiation under normoxia versus hypoxia. Radiation dose response curves of MDA-MB-231 wt (A, B), MDA-MB-231 EV (C, D) and MDA-MB-231 dnHIF (E, F) cells after 24 h treatment with 8 nM gemcitabine (dFdC) or 2 μM dFdU under normoxia (N) or hypoxia (H), immediately followed by radiation (RT) and reoxygenation. Survival curves were corrected for the cytotoxic effect of gemcitabine or dFdU alone and/or for loss of clonogenic capacity induced by exposure to hypoxia (see Table 2). All results are from at least 3 independent experiments ± SD.