Evaluation of interferon response, Cyclin D3, hTERT and caspase activity post TGS of
(A) No Significant induction of OAS1 levels were seen in HepG2, Huh7 and Chang Liver cells, post 5 days of virosomal delivery, by chimeric AFP promoter driven shRNA constructs, confirming the absence of interferon response (p > 0.05 at all point). (B) Decrease in the RNA level of both Cyclin D3 and hTERT (p = 0.0022 and p < 0.001 respectively) following c-myc suppression was seen in HepG2 cells through AFPEn–Pr + 2 – myc when compared to its control AFPEn–Pr + 2 – myc Scr. (C) Similar results were observed at the protein level. (D)
c-Myc suppression by AFPEn–Pr + 2 – myc, post 5 days after virosomal delivery, led to increase in caspase 3/7 activity in HepG2 cells (p = 0.005) and Huh7 cells (p = 0.035). However, increase in caspase 3/7 activity in Huh7 was to a lesser degree. No such increase was observed in the case of Chang Liver cell line (p = 0.38).