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Table 1 Primers used for gene expression

From: Molecular subtyping of metastatic melanoma based on cell ganglioside metabolism profiles

Gene Forward primer Reverse primer
GD3 synthase (ST8SIAI) 5′-CAGCATAATTCGGCAAAGGT-3′ 5′-ATTGGCACCAACATCTGACA-3′
NEU1 5′-CCTGGATATTGGCACTGAA-3′ 5′-CATCGCTGAGGAGACAGAAG-3′
NEU2 5′-AGAAGGATGAGCACGCAGA-3′ 5′-GGATGGCAATGAAGAAGAGG-3′
NEU3 5′-GGCTTGTTTGGGTGTTTGTT-3′ 5′-CATCGCTGAGGAGACAGAAG-3′
NEU4 5′-ACCGCCGAGAGTGTTTTGG-3′ 5′-CGTGGTCATCGCTGTAGAAGG-3′
GM3 synthase (ST3GAL5) 5′-CCCTGAACCAGTTCGATGTT-3′ 5′-CATTGCTTGAAGCCAGTTGA-3′
GM1 synthase (B3GALT4) 5′-CGCCTTCCAGGACTCCTACC-3′ 5′-CCGTCTTGAGGACGTATCGG-3′
GM2 synthase (B4GALNT1) 5′-TCTCACTGGAGAGGGTCAGG-3′ 5′-CGGACTGTGTCTGCTGTGTT-3′
GD1a synthase (ST6GALNAC4) 5′-TCTACCACCCAGCCTTCATC-3′ 5′-TAGTGGTGCCAGTTCCCTTT-3′
ST3GalVI 5′-TTGCCCTATGGGATGAGAAC-3′ 5′-CCTCCATTACCAACCACCAC-3′
ST3GalNac6 5′-CGCCGGAGAGAAATGAGTA-3′ 5′-CCACTTCTTGAGGTTGACAGG-3′
SP1 5′-GGCTACCCCTACCTCAAAGG-3′ 5′-CACAACATACTGCCCACCAG-3′
CMAH 5′-GAATTTATTGTCCCTTTGC-3′ 5′-TATGATGCCCTTGCTGTC-3′
CMAH deletion 5′-AGCTTTCCGGAGGGTTGATA-3′ 5′-TGTCAGATGCACAAGCACAA-3′
ACTB (β actin) 5′-CGACAGGATGCAGAAGGAG-3′ 5′-ACATCTGCTGGAAGGTGGA-3′