Table 1 Primers used for gene expression
From: Molecular subtyping of metastatic melanoma based on cell ganglioside metabolism profiles
Gene | Forward primer | Reverse primer |
|---|---|---|
GD3 synthase (ST8SIAI) | 5′-CAGCATAATTCGGCAAAGGT-3′ | 5′-ATTGGCACCAACATCTGACA-3′ |
NEU1 | 5′-CCTGGATATTGGCACTGAA-3′ | 5′-CATCGCTGAGGAGACAGAAG-3′ |
NEU2 | 5′-AGAAGGATGAGCACGCAGA-3′ | 5′-GGATGGCAATGAAGAAGAGG-3′ |
NEU3 | 5′-GGCTTGTTTGGGTGTTTGTT-3′ | 5′-CATCGCTGAGGAGACAGAAG-3′ |
NEU4 | 5′-ACCGCCGAGAGTGTTTTGG-3′ | 5′-CGTGGTCATCGCTGTAGAAGG-3′ |
GM3 synthase (ST3GAL5) | 5′-CCCTGAACCAGTTCGATGTT-3′ | 5′-CATTGCTTGAAGCCAGTTGA-3′ |
GM1 synthase (B3GALT4) | 5′-CGCCTTCCAGGACTCCTACC-3′ | 5′-CCGTCTTGAGGACGTATCGG-3′ |
GM2 synthase (B4GALNT1) | 5′-TCTCACTGGAGAGGGTCAGG-3′ | 5′-CGGACTGTGTCTGCTGTGTT-3′ |
GD1a synthase (ST6GALNAC4) | 5′-TCTACCACCCAGCCTTCATC-3′ | 5′-TAGTGGTGCCAGTTCCCTTT-3′ |
ST3GalVI | 5′-TTGCCCTATGGGATGAGAAC-3′ | 5′-CCTCCATTACCAACCACCAC-3′ |
ST3GalNac6 | 5′-CGCCGGAGAGAAATGAGTA-3′ | 5′-CCACTTCTTGAGGTTGACAGG-3′ |
SP1 | 5′-GGCTACCCCTACCTCAAAGG-3′ | 5′-CACAACATACTGCCCACCAG-3′ |
CMAH | 5′-GAATTTATTGTCCCTTTGC-3′ | 5′-TATGATGCCCTTGCTGTC-3′ |
CMAH deletion | 5′-AGCTTTCCGGAGGGTTGATA-3′ | 5′-TGTCAGATGCACAAGCACAA-3′ |
ACTB (β actin) | 5′-CGACAGGATGCAGAAGGAG-3′ | 5′-ACATCTGCTGGAAGGTGGA-3′ |