Figure 1

Enhanced signaling capacity of AB215 compared to BMP2 in breast cancer cells. A) Time course western blot analysis of MCF7 cells was performed after exposing BMP2 and AB215 for indicated time. The arrow points to the ligand-induced upper band. B-C) ID1-driven Luciferase reporter assay of BMP2 and AB215 for MCF7, T47D and SK-BR-3 cells in the B) absence and C) presence of 10nM E2. Cells were reverse transfected with ID1-Luciferase reporter plasmid in triplicate. Transfected cells were exposed to ligands for 18 hours. Relative luciferase value has been normalized with β-gal. The data is shown in means ± SD, (n: number of independent experiments = 3). D) mRNA level of IDs in MCF7 cells. BMP2 and AB215 induced mRNA expression level of ID1/2/3/4 has been measured by qRT-PCR in MCF7 cells. Cells were exposed to 500 ng/ml of BMP2 and AB215 for 18 hours. Relative mRNA level was calculated in quadruplicate using ΔΔCτ method with the control and β-actin as a reference. All data are shown in means + SD, n = 3. (* = P ≤ 0.05, ** = P ≤ 0.01, *** = P ≤ 0.001).