Figure 3

Dicer mRNA and protein expression after exposure to HIF hydroxylase inhibitors. Dicer mRNA and protein expression was examined in MCF7 and SKBR3 cells after exposure to HIF hydroxylase inhibitors: dimethyloxalyl glycine (DMOG) and desferrioxamine (DFO) A, Dicer mRNA expression (P = 0.04) in MCF7 cells after exposure to 1 mM DMOG for 48 h. B, Dicer mRNA expression in SKBR3 cells after exposure to 0.1 mM DFO for 48 h. *denotes P < 0.05 compared with parallel controls. Data represent normalized mean ± S.E (error bars) (n = 3). Dicer mRNA levels were analysed by RT-PCR and normalised to 18S rRNA levels. Statistical significance established by Student’s t-test. C, Dicer protein expression in MCF7 cells after exposure to 1 mM DMOG for 48 h Results show two technical replicates per treatment. D, Dicer protein expression in SKBR3 cells after exposure to DFO (0.1 mM) for 48 h. Results show three technical replicates per treatment. Dicer and α-actinin protein expression was examined by immunoblotting. α-actinin was used as the loading control.