Representative results for BRAF V600 mutation detection. Detection of V600E (A) and V600K (B) mutation using HRM. Normalized high-resolution melting curves (upper panel). The differences plot displays the melting curve of each tested sample subtracted from the reference curve obtained by analyzing a control wild-type BRAF sequence (below panel). Detection of V600E (C) and V600K (D) mutation using Sanger sequencing.