Figure 1

miRNAs significantly different in triple negative breast cancer. A) Supervised hierarchical cluster analysis was performed on 71 miRNAs significantly altered between tumour (n = 31, red branches, top) and normal adjacent tissue samples (n = 23, blue branches, top). Similarity in the expression patterns between miRNAs (branches shown on the left-hand panel) and between samples (branches shown on the top panel) was measured using Pearsons correlation. Distances between clustered branches represent the average distances between miRNAs and samples in the cluster. The height of each branch represents the degree of similarity within the cluster. miRNAs are coloured according to their expression level, where up-regulated expression is represented by red, down-regulated expression is represented by blue, and equal expression is represented by yellow. B) Relative quantification of miR-210, miR-100, miR-130a, let-7b and let-7c by real-time RT-PCR in normal (n = 24) and tumour samples (n = 35). Results are shown as a scatter plot of the relative normalised expression (target/RNU44) of the target miRNA (2^-ΔCt). Values represent the median ± interquartile range. *p < 0.0001, **p = 0.0004. C) Correlation between real-time PCR and miRNA array results. Relative quantification of fold change (IDC v NAT) in miR-210, miR-100, miR-130a, let-7b and let-7c between normal and tumour samples by real-time RT-PCR (2^-ΔΔCt, y-axis) or miRNA array (x-axis).