Figure 4

Restoration of gene and microRNA expression in GCTSCs after treatment with epigenetic modifiers. GCTSCs (n = 5) and MSCs (n = 5) were cultured in medium containing 10 μM 5-Aza-2′-deoxycytidine (Aza), 3 mM phenylbutyric acid (PBA) or both for 10 days. (A) Expression of Dlk1, Meg3, Meg8, Rtl1 and Dio3 normalized to the RPL19 expression in the corresponding sample. (B) Expression of miR-127-3p andmiR-376c normalized to the RNU6B expression in the corresponding sample. Data are presented as mean ± SD. (*p < 0.05 **p < 0.01 compared to untreated control cells determined by Mann–Whitney-U test).