Immunohistochemical staining of HLTF in benign thyroid lesions and statistical analysis of HLTF staining in a series of 4 groups of benign thyroid lesions. (A-D) A: colloid nodule, B: adenoma, C: Hashimoto’s Thyroiditis and D: Graves’ Disease. The scale bar is 50µm. (E-F) CN: colloid nodules, Ad: follicular adenomas HT: Hashimoto’s Thyroiditis and GD: Graves’ Diseases. The immunohistochemical expression was semi-quantitatively assessed in the nuclear compartment using (E) the LI (LI nuc) and (F) the MI (MI nuc). The threshold of significance associated with the global multigroup comparison (Kruskal-Wallis test) is located in the higher right corner. Horizontal arrows represent the post-hoc comparison for the pairs of groups, with corresponding p-values.