Figure 3

Signaling through the NOTCH1 pathway induces migration and an EMT phenotype in SHARP1 -overexpressing Ishikawa cells. (A) Western blot analysis of Ishikawa^SHARP1 cells expressing the intracellular domain of NOTCH1 (ICN) or a negative control (NC). Protein levels of ICN, HES1 and EMT-related markers were analyzed by western blotting in Ishikawa^SHARP1 cells (Left), and further quantified by densitometry of triplicate blots (right). β-actin was included as an internal control. (B) Trans-well migration assays involving Ishikawa^SHARP1 cells expressing ICN or NC. Representative images were obtained at 200× magnification. Graph shows the average number of migrated cells (n = 5 images) for the two treatment groups. Data represent the mean ± SD from one representative experiment of three independent experiments. **P < 0.01, ***P < 0.001.