Figure 2

SHARP1 overexpression reverses the EMT phenotype in EC cells. (A) SHARP1 overexpression induced an epithelial morphology in Ishikawa and HEC-1B cells (magnification: 200×). (B) Western blot analysis of SHARP1 and EMT-related markers in Ishikawa and HEC-1B cells (left), and blots were further quantified by densitometry of triplicate blots (right). β-actin was included as an internal control. (C) Western blot analysis of the effects of SHARP1 overexpression on NOTCH1 signaling components in Ishikawa and HEC-1B cells (left), and blots were further quantified by densitometry of triplicate blots (right). β-actin was included as an internal control. *P < 0.05, **P < 0.01, ***P < 0.001.