Figure 1

SHARP1 inhibits migration, invasion, and metastasis of EC cells. (A) Overexpression of SHARP1 in Ishikawa and HEC-1B cells revealed by qPCR (left) and western blotting (middle), and blots were further quantified by densitometry of triplicate blots (right), β-actin was included as an internal control. (B) Wound-healing assays for Ishikawa and HEC-1B cells. Representative images were obtained at 100× magnification. Graphs show the relative migration distance after 12 h incubation. (C) Trans-well invasion assays for Ishikawa and HEC-1B cells. Representative images were obtained at 200× magnification. Graph shows the number of invasive cells for each treatment group (averaged across five random images). (D) Ishikawa^Control or Ishikawa^SHARP1 cells were injected intravenously into SCID mice. Sections of lung tissue were stained with hematoxylin and eosin. (M) metastatic tumor, (N) normal lung tissue. Images were obtained at 40× (left) magnification. Graph shows the number of visible tumor nodules within analyzed lung tissue (n = 6 per group). Data represent the mean ± SD from one representative experiment of three independent experiments. **P < 0.01, ***P < 0.001.