Figure 1

Representative images of HCA2, HC10, HLA-E and –G and Foxp3+ staining in rectal cancer. Representative images of immunohistochemical stainings for HLA Class I expression (HCA2 and HC10), HLA-E and HLA-G expression and presence of Foxp3+ cells, performed according to standard protocols (details in Material and Methods). (A) HCA2-positive tumor (note: positive tumor cells in blue, stromal cells are stained brown); (B) HC10-positive tumor (note: positive tumor cells in blue, stromal cells are stained brown); (C) HLA-E positive tumor (note: positive tumor cells in brown); (D) HLA-G positive tumor (note: positive tumor cells in brown); (E) Presence of Foxp3+ cells (two representative examples of Foxp3+ cells are indicated by arrows) with a magnification in (F).