The expression profile of cancer-related markers in primary K9TCC cells. K9TCC cells were grown in presence and absence of serum for 24 hours and collected cell lysates were analyzed by WB. The expression levels of PDGFR, EGFR, VEGFR, p-ERK1/2, COX-2, p65, cyclin D1, and p27 were evaluated. Actin was used as loading control. The arrow shows the specific band for p65.