Table 1 Catalogue of the identified peptide targets commonly expressed in both MCF-Exo and MDA-Exo

Actin, cytoplasmic 1

41.7

ACTB

938.5

R.GYSFTTTAER.E

R.DLTDYLMK.I

R.DLTDYLMK.I

R.GYSFTTTAER.E

R.GYSFTTTAER.E

K.EITALAPSTMK.I

K.EITALAPSTMK.I

Annexin A2

38.6

ANXA2

563

K.AYTNFDAER.D

R.DALNIETAIK.T

K.TPAQYDASELK.A

K.TPAQYDASELK.A

K.DIISDTSGDFR.K

R.TNQELQEINR.V

R.TNQELQEINR.V

R.TNQELQEINR.V

R.TNQELQEINR.V

Pyruvate kinase isozymes M1/M2

57.9

KPYM

340

K.GDYPLEAVR.M

K.DITSDTSGDFR.N

K.TPAQFDADELR.A.K

Tubulin beta chain

49.6

TBB5

258.5

K.LAVNMVPFPR.L

K.EVDEQMLNVQNK.N

Heat shock cognate 71 kDa protein

70.8

HSP7C

252.5

K.DAGTIAGLNVLR.I

K.NQVAMNPTNTVFDAK.R

Glyceraldehyde-3-phosphate dehydrogenase

36

G3P

249.5

R.VVDLMAHMASK.E

K.LISWYDNEFGYSNR.V

Tubulin alpha-1C chain

49.8

TBA1C

223.5

K.DVNAAIATIK.T

K.TIGGGDDSFNTFFSETGAGK.H

Sodium/potassium-transporting ATPase subunit alpha-1

11.2

AT1A1

197

K.TSATWLALSR.I

R.LNIPVSQVNPR.D

14-3-3 protein zeta/delta

27.7

1433Z

194.5

R.YLAEVAAGDDKK.G

R.YLAEVAAGDDKK.G

K.SVTEQGAELSNEER.N

K.GIVDQSQQAYQEAFEISK.K

Histone H4

11.3

H4

181.5

R.ISGLIYEETR.G

K.TVTAMDVVYALK.R

R.DNIQGITKPAIR.R

R.DNIQGITKPAIR.R

K.TVTAMDVVYALK.R

R.KTVTAMDVVYALK.R

Heat shock protein HSP 90-alpha

84.6

HS90A

167.5

K.DQVANSAFVER.L

R.ELISNSSDALDK.I

K.EDQTEYLEER.R

R.GVVDSEDLPLNISR.E

R.NPDDITNEEYGEFYK.S

Heat shock protein HSP 90-beta

83.2

HS90B

156

K.EQVANSAFVER.V

K.EDQTEYLEER.R

R.GVVDSEDLPLNISR.E

R.NPDDITQEEYGEFYK.S

Brain acid soluble protein 1

22.6

BASP1

155.5

K.AEPPKAPEQEQAAPGPAAGGEAPK.A

K.AEPPKAPEQEQAAPGPAAGGEAPK.A

K.AAEAAAAPAESAAPAAGEEPSKEEGEPK.K

K.AQGPAASAEEPKPVEAPAANSDQTVTVKE

Elongation factor 1-alpha 1

50.1

EF1A1

128.5

K.IGGIGTVPVGR.V

K.STTTGHLIYK.C

K.STTTGHLIYK.C

K.STTTGHLIYK.C

K.STTTGHLIYK.C

Myristoylated alanine-rich C-kinase substrate

31.5

MARCS

118

K.EAPAEGEAAEPGSPTAAEGEAASAASSTSSPK.A

K.EELQANGSAPAADKEEPAAAGSGAASPSAAEK.G

Annexin A5

35.9

ANXA5

98

K.VLTEIIASR.T

R.SEIDLFNIR.K

Calmodulin

16.8

CALM

96.5

K.EAFSLFDKDGDGTITTK.E

R.VFDKDGNGYISAAELR.H

MARCKS-related protein

19.5

MRP

90

K.GEGESPPVNGTDEAAGATGDAIEPAPPSQGAEAK.G

R.GDVTAEEAAGASPAK.A

Integrin alpha-2

129.2

ITA2

76

K.TQVGLIQYANNPR.V

Galectin-3-binding protein

65.3

LG3BP

73

R.ASHEEVEGLVEK.I

Histone H1t

22

H1T

60.5

K.ALAAAGYDVEK.N

Alpha-enolase

47.1

ENOA

60.5

R.YISPDQLADLYK.S

Kinesin-like protein KIF12

70.6

KIF12

60

K.LTKLLADSLGGR.G

Elongation factor 1-gamma

50.1

EF1G

57

K.ALIAAQYSGAQVR.V

Peroxiredoxin-1

22.1

PRDX1

50.5

K.ATAVMPDGQFK.D

R.QITVNDLPVGR.S

Ubiquitin-40S ribosomal protein S27a

17.9

RS27A

49.5

K.ESTLHLVLR.L

14-3-3 protein epsilon

29.1

1433E

43.5

K.EAAENSLVAYK.A