Figure 5
From: Tyrosine phosphorylation profiling via in situproximity ligation assay
In situ PLA for pTyr proteins applied to the micro array format. Formalin fixed and paraffin embedded K562 (Panel A) and U937 (Panel B) cells assembled in a micro array format reacted with 4G10 antibody in in situ PLA to visualize the pTyr modification. Red dots represent pTyr signals while nuclei and cytoplasms are stained in blue and green, respectively. Scale bars 10 μm.