Cell signaling changes induced by platelets. (A) Analysis of changes in p-ERK, p-JNK, p-STAT3, p-p38 and p-AKT. PLC/PRF/5 cells were treated with 3.75 × 107 PLTs or FBS for different times. The changes in protein expression were evaluated using Western blot (WB) analysis. (B) The Western blot (WB) analysis confirmed the anti-apoptotic effect of platelets as showed in Figure 2C. (C) To test the significance of p-JNK increase, observed in WB analysis (A), on platelet-mediated growth induction, SP600125 (JNK inhibitor) was used, resulting in inhibition of PLT action. *P < 0.05.