Effects of platelet extracts on HCC cell line growth. (A) Comparison of serum concentrations on platelet actions. PLC/RFP/5 cells were cultured in different FBS percentage (0-5%) for 48 h in presence of PLTs or equivalent FBS concentration (control). Performing MTT assay, a significant difference in growth is detected only at 1% FBS. (B-E) Effects of platelets on the growth of different human HCC cell lines. PLC/PRF/5 (B), HepG2 (C), Hep3B (D) and Huh7 (E) cell lines were cultured in 1% FBS medium in presence of different platelets concentrations or FBS and MTT assay was assessed after 48 h. (F) Comparison of different concentrations of red cells (RBC), white cells (WBC) and platelets on PLC/PRF/5 cell growth evaluated after 48 h by MTT assay. (G) Comparison of time windows (0-24 h, 0-48 h, 48-72 h, 24-72 h and 0-72 h) for effects of two different platelet concentrations on growth evaluated after 72 h by MTT assay. The results are expressed as mean ± SD. *P < 0.05; **P < 0.001; ***P < 0.0001.