Effect of celecoxib and aspirin on plasma estradiol concentration, uterine weight and ERα expression. Blood was drawn from the animals at sacrifice. Serum estradiol concentration was quantified by ELISA (A). Sample labeled as AD is androstenedione. Uterine of the experimental animals were dissected and weighed at sacrifice as shown in B. Western blot analysis of ERα in xenograft samples was analyzed by immunoblotting (C) and the optical density measurements were shown in the lower panel. Groups labeled as AD, AD+celecoxib, and AD+aspirin are the mice treated with androstenedione, androstenedione and celecoxib, and androstenedione and aspirin, respectively. Values are means ± SEM, n=6 to 8.The data was analyzed by One-way ANOVA, followed by Tukey’s Multiple Comparison test when P<0.05. Means labeled with different letter are significantly different (order: b>a).