Figure 2

Effects of BCL6 on regulation of breast cancer cell phenotype. (a) Cell viability MTT assay. Cells were transiently transfected with BCL6 siRNA vs. negative control (NC) or BCL6 cDNA vs. control vector (VEC), respectively and then seeded in 96-well plates (3 × 10³ per well) and grown for 4 days for MTT assay. (b) Wound healing assay. T47D cells were grown and transiently transfected with BCL6 siRNA or negative control (NC), the wounded monolayers were cultured in the absence (left) or presence (right) of mitomycin C. (c) Flow cytometric analysis of cell cycle distribution in MCF-7 cells after gene transfection. (d) Flow cytometric analysis of apoptosis in MCF-7 cells after gene transfection. The average of apoptosis rate is presented as mean ± SD. All experiments were repeated at least three times. **P < 0.01.