Figure 7
Effect of PGE2 and forskolin on TGFβ-stimulated collagen synthesis. A, B. Effects of different concentrations of PGE2 on TGFβ-stimulated collagen synthesis. Cells were stimulated for 48 hours. Results are presented as mean ± S.E.M. of six experiments. C. Effect of forskolin on TGFβ stimulated collagen synthesis. Cells were stimulated for 48 hours. Results are presented as mean ± S.E.M of five experiments. D. Effect of TGFβ (10 ng/ml) alone and in combination with 100 nM PGE2 on collagen 1A1 mRNA expression. Cells were stimulated for 24 hours before RNA was isolated and real time quantitative RT-qPCR was performed. Results are presented as mean ± S.E.M. of eight experiments. E. Effect of TGFβ (10 ng/ml) alone and in combination with 1 μM PGE2 on collagen 1A1 mRNA expression. Cells were stimulated for 24 hours before RNA was isolated and real time quantitative RT-qPCR was performed. Results are presented as mean + S.E.M. of three experiments. * Sign. different from control ** Sign. different from TGFβ alone. F. Effect of the EP4 receptor antagonist ( L161982 1 μM) on PGE2 influence on TGFβ-stimulated collagen synthesis. Cells were stimulated for 48 hours. Results are presented as mean ± S.E.M. of three experiments. *Sign. different from control ** Sign. different from TGFβ alone.