Figure 2

Top 20 hypoxia-regulated genes according to P -values and validation of microarray results using quantitative PCR. (A) Only significantly regulated genes with a minimum fold-change of 2 are included. The analysis was performed for all samples (n = 20 patients) or for adenocarcinoma (n = 10 patients) and squamous cell carcinoma (n = 10 patients) separately. Overlapping genes, regulated in the same direction in both histological subtypes are highlighted. (B) Confirmation of the up-regulation of overlapping hypoxia-regulated genes in hypoxic and normoxic fragments derived from NSCLC surgical specimens from four patients by quantitative PCR. Results are shown as mean +/-SEM. No difference in expression gives a value of zero. Significance was calculated with one-sample Student’s t-test. HK2, hexokinase 2; MME, membrane metallo-endopeptidase; KCTD11, potassium channel tetramerisation domain containing 11; PPP1R3C, protein phosphatase 1 regulatory subunit 3C; FAM115C, family with sequence similarity 115 member C. *P < 0.05, **P < 0.01.